彈性蛋白酶 Elastase
彈性蛋白酶 Elastase 來源於(yu) 豬胰髒,分子質量25.9KD,PH8.5.彈性蛋白酶水解的蛋白底物的種類繁多,但也有其*性,它能夠水解彈性蛋白,胰蛋白酶,糜蛋白酶或胃蛋白酶不能作用的底物。大豆胰蛋白酶抑製劑和激肽釋放酶抑製劑抑製蛋白水解,但無彈性蛋白離解活性。
穩定性/儲(chu) 存:彈性蛋白酶在pH值≤3.5時不穩定。2-8° C幹粉穩定貯存6-12個(ge) 月。
彈性蛋白酶產(chan) 品代碼:ES和ESL在中性pH值和在濃度大於(yu) 0.25%時溶解性差。當用反應混合物或培養(yang) 基稀釋彈性蛋白酶之前,調整kcl或堿性緩衝(chong) 液的離子強度或PH值有助於(yu) 彈性蛋白酶的溶解,當pH4.0-10.4時穩定。
技術說明:1 SucAla3NA單位大約相當於(yu) 6倍的彈力蛋白酶的消化單位。應用無菌水進行溶解,以避免細菌汙染。由於(yu) 懸浮液的粘稠性(代號:ES的小瓶包裝)應用水衝(chong) 洗以保持濃度。
單位定義(yi) :25 ° C,pH值8.0,1單位的酶每分鍾切開1微摩爾的N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide的量。
Description | Activity | Code | Size | Cat. No. | Price | ||
---|---|---|---|---|---|---|---|
Elastase, Purified Chromatographically purified. A lyophilized powder. Store at 2-8° C. REQUIRES SPECIAL SHIPPING: ICE PACK | ≥8 units per mg protein | ESFF | 5 mg | LS006363 | |||
20 mg | LS006365 | ||||||
Bulk | LS006367 | Inquire | |||||
Elastase, Lyophilized 2X Crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells. Store at 2-8° C. | ≥3 units per mg protein | ESL | 25 mg | LS002290 | |||
100 mg | LS002292 | ||||||
1 gm | LS002294 | ||||||
Bulk | LS002298 | Inquire | |||||
Elastase, Suspension 2X crystallized. Supplied as an aqueous suspension. This preparation must be diluted to dissolve the enzyme. The diluted enzyme should be 0.22 micron filtered before use. Suitable for the isolation of Type II lung cells. Store at 2-8° C. DO NOT FREEZE REQUIRES SPECIAL SHIPPING: ICE PACK. | ≥3 units per mg protein | ES | 25mg | LS002274 | |||
100mg | LS002279 | ||||||
1 gm | LS002280 | ||||||
Bulk | LS002276 | Inquire |
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